Journal: Practical Laboratory Medicine

Article Title: Fluorimetric assay with a novel substrate for quantification of galactocerebrosidase activity in dried blood spot specimens

doi: 10.1016/j.plabm.2019.e00141

Figure Lengend Snippet: Buffer optimization . Pooled extracts from QCH and PT dried blood spots (A) or recombinant human GALC and β-galactosidase (B) were tested for GALC activity in the presence (grey) or absence (black) of 150 mmol/l NaCl to assess the effect of salt on measured enzyme activity. The graphs show mean enzyme activities for each sample tested in duplicate, with error bars representing the standard deviations from the mean.

Article Snippet: Recombinant human galactocerebrosidase (rhGALC) and recombinant human β-galactosidase (rhβ-gal) were obtained from R&D Systems, Inc. (Minneapolis, MN).

Techniques: Recombinant, Activity Assay

Journal: Practical Laboratory Medicine

Article Title: Fluorimetric assay with a novel substrate for quantification of galactocerebrosidase activity in dried blood spot specimens

doi: 10.1016/j.plabm.2019.e00141

Figure Lengend Snippet: Specificity of GALC assay conditions. (A–B) Pooled extracts from quality control high (QCH; grey) and proficiency testing deficient (PT; black) samples were tested using two different assay conditions optimized to measure activity of β-galactosidase (A) or GALC (B). The graphs show mean enzyme activities for each sample tested in duplicate, with error bars representing the standard deviations from the mean.

Article Snippet: Recombinant human galactocerebrosidase (rhGALC) and recombinant human β-galactosidase (rhβ-gal) were obtained from R&D Systems, Inc. (Minneapolis, MN).

Techniques: Activity Assay

Journal: Biomaterials

Article Title: Tissue engineering the mechanosensory circuit of the stretch reflex arc with human stem cells: Sensory neuron innervation of intrafusal muscle fibers

doi: 10.1016/j.biomaterials.2017.01.005

Figure Lengend Snippet: Composition of enriched Co-culture media (medium 2).

Article Snippet: Thereafter, the cultures were fed every 2 days using NBac-tive4 (Brain Bits, Nb4-500) by replacing half of the medium. table ft1 table-wrap mode="anchored" t5 caption a7 Component Full name Concentration Company Catalog number Neurobasal/Neurobasal A Invitrogen 10888/21103 B27 (50X) 1X Invitrogen 17504–044 Glutamax (100X) 1X Invitrogen 35050 rhNRG Neuregulin-1-β1 EGF domain 100 ng/ml R&D 396-GF/CF Rhβ-NGF Nerve Growth Factor 100 ng/ml R&D 256-GF/CF GDNF Glial-derived Neurotrophic Factor 10 ng/ml Cell Sciences CRG400B BDNF Brain-derived Neurotrophic Factor 20 ng/ml Cell Sciences CRB600B Shh Sonic Hedgehog, N-terminal peptide 50 ng/ml R&D 1845-SH-025 RA Retinoic Acid 0.1 uM Sigma R2625 IGF-1 Insulin-like Growth Factor -I 10 ng/ml PeproTech 100–11 cAMP Adenosine 3′,5′-cyclic Monophosphate 1 uM Sigma A9501 CNTF Ciliary Neurotrophic Factor 5 ng/ml Cell Sciences CRC400A NT-3 Neurotrophin-3 20 ng/ml Cell Sciences CRN500B NT-4 Neurotrophin-4 20 ng/ml Cell Sciences CRN501B Vitronectin 100 ng/ml Sigma V8379 Laminin Mouse Laminin 4 μg/ml Invitrogen 23017–015 G5 (100X) 1X Invitrogen 17503–012 Agrin 100 ng/ml R&D 550-AG-100 Open in a separate window Composition of enriched Co-culture media (medium 2).

Techniques: Concentration Assay